In the Raw Data, several columns such as mean Ct, or delta Ct are listed. These values do not have any relevance for the here performed analysis and reported results. 
These values are generated automatically by the software. 
However, we have not used this automatic calculation. Neither control samples, nor the housekeeping genes were identified as such in the software.

Ct values were exported as indicated in the spreadsheet "PCR_Sample assignment" and analysed manually. 

Please also see the excel spreadsheet "PCR_sample assignment" for sample identification code and assignment of individual replicates. 